Antigen

Antigen Class

TFs and others

Antigen

MITF

Cell type

Cell type Class

Muscle

Cell type

SU-CCS-1

Tissue

Soft Tissue

Cell Type

Clear Cell Sarcoma Cell Line

Disease

Clear Cell Sarcoma

Sample

source_name

Cell line

cell type

Derived from the pleural effusion of a 16 year old Caucasian girl with a heel CCS tumor

chip antibody

MITF (#91201, Active motif)

treatment

Transfected with CTRL siRNA

cell line

SU-CCS-1 ATCC CRL-2971

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were cross-linked with formaldehyde, flash-frozen and processed for ChIP using Protein G beads. ChIP DNA was purified with AMPure XP beads. ChIP DNA libraries were prepared using the Illumina TruSeq ChIP library preparation kit (Illumina cat. no. IP-202-1012 or IP-202-1024) according to the manufacturer's instructions and 200-500 bp fragments selected using AMPureXP beads.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

29312804

Reads aligned (%)

98.3

Duplicates removed (%)

16.8

Number of peaks

80499 (qval < 1E-05)

hg19

Number of total reads

29312804

Reads aligned (%)

97.8

Duplicates removed (%)

17.3

Number of peaks

80101 (qval < 1E-05)